Performance of plate-based cytokine flow cytometry with automated data analysis

BACKGROUND: Cytokine flow cytometry (CFC) provides a multiparameter alternative to ELISPOT assays for rapid quantitation of antigen-specific T cells. To increase the throughput of CFC assays, we have optimized methods for stimulating, staining, and acquiring whole blood or PBMC samples in 96-well or...

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Bibliografiske detaljer
Main Authors: Suni, Maria A, Dunn, Holli S, Orr, Patricia L, Laat, Rian de, Sinclair, Elizabeth, Ghanekar, Smita A, Bredt, Barry M, Dunne, John F, Maino, Vernon C, Maecker, Holden T
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2003
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC200973/
https://ncbi.nlm.nih.gov/pubmed/12952557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-4-9
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