Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT

BACKGROUND: Cryopreservation of PBMC and/or overnight shipping of samples are required for many clinical trials, despite their potentially adverse effects upon immune monitoring assays such as MHC-peptide tetramer staining, cytokine flow cytometry (CFC), and ELISPOT. In this study, we compared the p...

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Detalhes bibliográficos
Main Authors: Maecker, Holden T, Moon, James, Bhatia, Sonny, Ghanekar, Smita A, Maino, Vernon C, Payne, Janice K, Kuus-Reichel, Kristine, Chang, Jennie C, Summers, Amanda, Clay, Timothy M, Morse, Michael A, Lyerly, H Kim, DeLaRosa, Corazon, Ankerst, Donna P, Disis, Mary L
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2005
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1190174/
https://ncbi.nlm.nih.gov/pubmed/16026627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-6-17
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