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Kinetically Competent Intermediate(s) in the Translocation Step of Protein Synthesis
Translocation requires large-scale movements of ribosome-bound tRNAs. Using tRNAs that are proflavin-labeled and single turnover rapid kinetics assays, we identify one or possibly two kinetically competent intermediates in translocation. EF-G.GTP binding to the pretranslocation (PRE) complex and GTP...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2007
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1995019/ https://ncbi.nlm.nih.gov/pubmed/17317625 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2007.01.014 |
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