Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate

Dihydroorotate dehydrogenase (DHOD) catalyzes the oxidation of dihydroorotate to orotate, the fourth step and the only redox reaction in the de novo biosynthesis of pyrimidine. DHOD from Trypanosoma cruzi (TcDHOD) has been expressed as a recombinant protein in Escherichia coli and purified to homoge...

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Bibliographic Details
Main Authors: Inaoka, Daniel Ken, Takashima, Eizo, Osanai, Arihiro, Shimizu, Hironari, Nara, Takeshi, Aoki, Takashi, Harada, Shigeharu, Kita, Kiyoshi
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2005
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1991314/
https://ncbi.nlm.nih.gov/pubmed/16511183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910502659X
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