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Removing the regulatory N-terminal domain of cardiac troponin I diminishes incompatibility during bacterial expression
Troponin I (TnI) is a muscle-specific protein and plays an allosteric function in the Ca(2+) regulation of cardiac and skeletal muscle contraction. Expression of cloned cDNA in E. coli is an essential approach to preparing human TnI and mutants for structural and functional studies. The expression l...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1991298/ https://ncbi.nlm.nih.gov/pubmed/17303066 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2007.01.011 |
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