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Overexpression, purification and crystallization of an archaeal DNA ligase from Pyrococcus furiosus

DNA ligases seal single-strand breaks in double-stranded DNA and their function is essential to maintain the integrity of the genome during various aspects of DNA metabolism, such as replication, excision repair and recombination. DNA-strand breaks are frequently generated as reaction intermediates...

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Bibliografiske detaljer
Main Authors: Nishida, Hirokazu, Tsuchiya, Daisuke, Ishino, Yoshizumi, Morikawa, Kosuke
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2005
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1978145/
https://ncbi.nlm.nih.gov/pubmed/16511246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105038649
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