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Intermolecular ion pairs maintain the toroidal structure of Pyrococcus furiosus PCNA

Two mutant proliferating cell nuclear antigens from the hyperthermophilic archaeon Pyrococcus furiosus, PfuPCNA(D143A) and PfuPCNA(D143A/D147A), were prepared by site-specific mutagenesis. The results from gel filtration showed that mutations at D143 and D147 drastically affect the stability of the...

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Main Authors: Matsumiya, Shigeki, Ishino, Sonoko, Ishino, Yoshizumi, Morikawa, Kosuke
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 2003
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2323854/
https://ncbi.nlm.nih.gov/pubmed/12649440
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