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Analysis of Read-Length Limiting Factors in Pyrosequencing Chemistry

Pyrosequencing is a bioluminometric DNA sequencing technique that measures the release of pyrophosphate during DNA synthesis. The amount of pyrophosphate is proportionally converted into visible light by a cascade of enzymatic reactions. Pyrosequencing has thus far been used for generating short seq...

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Detalhes bibliográficos
Main Authors: Mashayekhi, Foad, Ronaghi, Mostafa
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1978072/
https://ncbi.nlm.nih.gov/pubmed/17343818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.02.002
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