Multiplex Pyrosequencing

We describe here the development of a new and simple single-tube multiplex Pyrosequencing assay. Genomic DNA or cDNA was employed to PCR amplify region(s) using biotinylated and normal primer(s). Subsequent to capture of PCR products on streptavidin-coated beads, single-stranded DNA separation and h...

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Bibliografske podrobnosti
Main Authors: Pourmand, Nader, Elahi, Elahe, Davis, Ronald W., Ronaghi, Mostafa
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2002
Teme:
NAR Methods Online
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC101855/
https://ncbi.nlm.nih.gov/pubmed/11917037
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