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Multiplex Pyrosequencing

We describe here the development of a new and simple single-tube multiplex Pyrosequencing assay. Genomic DNA or cDNA was employed to PCR amplify region(s) using biotinylated and normal primer(s). Subsequent to capture of PCR products on streptavidin-coated beads, single-stranded DNA separation and h...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Pourmand, Nader, Elahi, Elahe, Davis, Ronald W., Ronaghi, Mostafa
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2002
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC101855/
https://ncbi.nlm.nih.gov/pubmed/11917037
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