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Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Kim, S W, Kim, C Y, Benisek, W F, Choi, K Y
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1994
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC197024/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7961420/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.21.6672-6676.1994
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