Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida...

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Pubblicato in:	J Bacteriol
Autori principali:	Kim, S W, Kim, C Y, Benisek, W F, Choi, K Y
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1994
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC197024/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7961420/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.21.6672-6676.1994
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