Direct screening of recombinants in gram-positive bacteria using the secreted staphylococcal nuclease as a reporter.

A system for direct screening of recombinant clones in Lactococcus lactis, based on secretion of the staphylococcal nuclease (SNase) in the organism, was developed. The nuc gene (encoding SNase) was cloned on both rolling-circle and theta-replicating plasmids. L. lactis strains containing these nuc+...

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Библиографические подробности

Опубликовано в::	J Bacteriol
Главные авторы:	Le Loir, Y, Gruss, A, Ehrlich, S D, Langella, P
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1994
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC196356/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8051029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.16.5135-5139.1994
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