Direct screening of recombinants in gram-positive bacteria using the secreted staphylococcal nuclease as a reporter.

A system for direct screening of recombinant clones in Lactococcus lactis, based on secretion of the staphylococcal nuclease (SNase) in the organism, was developed. The nuc gene (encoding SNase) was cloned on both rolling-circle and theta-replicating plasmids. L. lactis strains containing these nuc+...

Volledige beschrijving

Bibliografische gegevens

Gepubliceerd in:	J Bacteriol
Hoofdauteurs:	Le Loir, Y, Gruss, A, Ehrlich, S D, Langella, P
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1994
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC196356/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8051029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.16.5135-5139.1994
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!