Direct screening of recombinants in gram-positive bacteria using the secreted staphylococcal nuclease as a reporter.

A system for direct screening of recombinant clones in Lactococcus lactis, based on secretion of the staphylococcal nuclease (SNase) in the organism, was developed. The nuc gene (encoding SNase) was cloned on both rolling-circle and theta-replicating plasmids. L. lactis strains containing these nuc+...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Le Loir, Y, Gruss, A, Ehrlich, S D, Langella, P
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1994
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC196356/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8051029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.16.5135-5139.1994
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