Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions.
An approach to analyzing the topology of membrane proteins with alkaline phosphatase fusions is described. Precise fusions were constructed by using polymerase chain reaction at the C terminus of each hydrophilic region of the membrane protein. The disruption of topogenic signals is thereby minimize...
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| Veröffentlicht in: | J Bacteriol |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1993
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC196172/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8419303/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.2.553-556.1993 |
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