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Use of gene fusions to determine a partial signal sequence of alkaline phosphatase.

We have isolated strains of Escherichia coli in which an amino-terminal portion of the cytoplasmic enzyme beta-galactosidase is replaced by an amino-terminal portion of the periplasmic enzyme alkaline phosphatase. The synthesis of these hybrid proteins is regulated by inorganic phosphate and they ar...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Sarthy, A, Fowler, A, Zabin, I, Beckwith, J
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1979
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218041/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/113391/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.139.3.932-939.1979
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