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Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

A phoA-lacZ gene fusion was used to isolate mutants altered in the alkaline phosphatase signal sequence. This was done by selecting Lac+ mutants from a phoA-lacZ fusion strain that produces a membrane-bound hybrid protein and is unable to grow on lactose. Two such mutant derivatives were characteriz...

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Autori principali: Michaelis, S, Inouye, H, Oliver, D, Beckwith, J
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1983
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC217468/
https://ncbi.nlm.nih.gov/pubmed/6339478
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