Genetic analysis of membrane protein topology by a sandwich gene fusion approach.
We describe a cloning vector that allows the construction of phoA sandwich fusions in which mature alkaline phosphatase is inserted into target proteins. In contrast to previous fusions obtained using the TnphoA transposon, the entire amino acid sequence of the target protein is present in the fusio...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54790/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2170984/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.19.7574 |
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