Fluorescence Lifetime Imaging to Detect Actomyosin States in Mammalian Muscle Sarcomeres

We investigated the use of fluorescence lifetime imaging microscopy (FLIM) of a fluorescently labeled ATP analog (3′-O-{N-[3-(7-diethylaminocoumarin-3-carboxamido)propyl]carbamoyl}ATP) to probe in permeabilized muscle fibers the changes in the environment of the nucleotide binding pocket caused by i...

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Autors principals: García, Delisa I., Lanigan, Peter, Webb, Martin, West, Timothy G., Requejo-Isidro, Jose, Auksorius, Egidijus, Dunsby, Chris, Neil, Mark, French, Paul, Ferenczi, Michael A.
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2007
Matèries:
Muscle and Contractility
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1959533/
https://ncbi.nlm.nih.gov/pubmed/17496049
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.096479
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