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Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes

Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To study calcium-triggered secretion from astrocytes, we used acridine orange (AO) to label vesicles. Although AO is often used for imaging exocytosis, we found that im...

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Detalhes bibliográficos
Main Authors: Jaiswal, Jyoti K., Fix, Marina, Takano, Takahiro, Nedergaard, Maiken, Simon, Sanford M.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1955787/
https://ncbi.nlm.nih.gov/pubmed/17715060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0704935104
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