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Genetic selection for a highly functional cysteine-less membrane protein using site-saturation mutagenesis
We describe an efficient method for generating highly functional membrane proteins with variant amino acids at defined positions that couples a modified site-saturation strategy with functional genetic selection. We applied this method to the production of a cysteine-less variant of the Crithidia fa...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1949046/ https://ncbi.nlm.nih.gov/pubmed/17481563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.03.030 |
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