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tRNA-modifying MiaE protein from Salmonella typhimurium is a nonheme diiron monooxygenase
MiaE catalyzes the posttranscriptional allylic hydroxylation of 2-methylthio-N-6-isopentenyl adenosine in tRNAs. The Salmonella typhimurium enzyme was heterologously expressed in Escherichia coli. The purified enzyme is a monomer with two iron atoms and displays activity in in vitro assays. The type...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1948905/ https://ncbi.nlm.nih.gov/pubmed/17679698 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0704338104 |
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