Semiquantitative Detection by Real-Time PCR of Aspergillus fumigatus in Bronchoalveolar Lavage Fluids and Tissue Biopsy Specimens from Patients with Invasive Aspergillosis

A real-time PCR method was developed and used to detect Aspergillus fumigatus mitochondrial DNA (mtDNA) in bronchoalveolar lavage (BAL) fluids and tissue biopsy specimens. The analytical sensitivity of the assay was one A. fumigatus conidium per reaction, and the assay was linear at least over 4 ord...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Rantakokko-Jalava, Kaisu, Laaksonen, Sanna, Issakainen, Jouni, Vauras, Jukka, Nikoskelainen, Jukka, Viljanen, Matti K., Salonen, Juha
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Mycology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193834/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12958261/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.9.4304-4311.2003
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