Semiquantitative Detection by Real-Time PCR of Aspergillus fumigatus in Bronchoalveolar Lavage Fluids and Tissue Biopsy Specimens from Patients with Invasive Aspergillosis

A real-time PCR method was developed and used to detect Aspergillus fumigatus mitochondrial DNA (mtDNA) in bronchoalveolar lavage (BAL) fluids and tissue biopsy specimens. The analytical sensitivity of the assay was one A. fumigatus conidium per reaction, and the assay was linear at least over 4 ord...

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Bibliographic Details

Published in:	J Clin Microbiol
Main Authors:	Rantakokko-Jalava, Kaisu, Laaksonen, Sanna, Issakainen, Jouni, Vauras, Jukka, Nikoskelainen, Jukka, Viljanen, Matti K., Salonen, Juha
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2003
Subjects:	Mycology
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193834/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12958261/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.9.4304-4311.2003
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