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Semiquantitative Detection by Real-Time PCR of Aspergillus fumigatus in Bronchoalveolar Lavage Fluids and Tissue Biopsy Specimens from Patients with Invasive Aspergillosis

A real-time PCR method was developed and used to detect Aspergillus fumigatus mitochondrial DNA (mtDNA) in bronchoalveolar lavage (BAL) fluids and tissue biopsy specimens. The analytical sensitivity of the assay was one A. fumigatus conidium per reaction, and the assay was linear at least over 4 ord...

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Autors principals: Rantakokko-Jalava, Kaisu, Laaksonen, Sanna, Issakainen, Jouni, Vauras, Jukka, Nikoskelainen, Jukka, Viljanen, Matti K., Salonen, Juha
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2003
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC193834/
https://ncbi.nlm.nih.gov/pubmed/12958261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.9.4304-4311.2003
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