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Two-Color Fluorescence Analysis of Individual Virions Determines the Distribution of the Copy Number of Proteins in Herpes Simplex Virus Particles

We present a single virion method to determine absolute distributions of copy number in the protein composition of viruses and apply it to herpes simplex virus type 1. Using two-color coincidence fluorescence spectroscopy, we determine the virion-to-virion variability in copy numbers of fluorescentl...

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Autors principals: Clarke, Richard W., Monnier, Nilah, Li, Haitao, Zhou, Dejian, Browne, Helena, Klenerman, David
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1929035/
https://ncbi.nlm.nih.gov/pubmed/17513380
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.106351
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