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Quantification of Fluorophore Copy Number from Intrinsic Fluctuations during Fluorescence Photobleaching
We present a theoretical technique for quantifying the cellular copy-number of fluorophores that relies on the random nature of the photobleaching process. Our approach does not require single-molecule sensitivity, and therefore can be used with commonly used epifluorescence microscopes. Fluctuation...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3207165/ https://ncbi.nlm.nih.gov/pubmed/22067169 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.09.032 |
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