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Fluorescence Fluctuation Spectroscopy in the Presence of Immobile Fluorophores

Fluorescence contributions from immobile sources present a challenge for fluorescence fluctuation spectroscopy (FFS) because the absence of signal fluctuations from stationary fluorophores leads to a biased analysis. This is especially of concern for cellular FFS studies on proteins that interact wi...

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Bibliographic Details
Main Authors: Skinner, Joseph P., Chen, Yan, Müller, Joachim D.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2008
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2257913/
https://ncbi.nlm.nih.gov/pubmed/18065480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.115642
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