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Characterization of Brightness and Stoichiometry of Bright Particles by Flow-Fluorescence Fluctuation Spectroscopy

Characterization of bright particles at low concentrations by fluorescence fluctuation spectroscopy (FFS) is challenging, because the event rate of particle detection is low and fluorescence background contributes significantly to the measured signal. It is straightforward to increase the event rate...

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Bibliografiske detaljer
Main Authors: Johnson, Jolene, Chen, Yan, Mueller, Joachim D.
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966004/
https://ncbi.nlm.nih.gov/pubmed/21044607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.057
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