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Characterization of Brightness and Stoichiometry of Bright Particles by Flow-Fluorescence Fluctuation Spectroscopy

Characterization of bright particles at low concentrations by fluorescence fluctuation spectroscopy (FFS) is challenging, because the event rate of particle detection is low and fluorescence background contributes significantly to the measured signal. It is straightforward to increase the event rate...

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Detalhes bibliográficos
Main Authors: Johnson, Jolene, Chen, Yan, Mueller, Joachim D.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966004/
https://ncbi.nlm.nih.gov/pubmed/21044607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.057
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