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Spectroscopic and Structural Study of Proton and Halide Ion Cooperative Binding to GFP
This study reports the influence of halogens on fluorescence properties of the Aequorea victoria Green Fluorescent Protein variant S65T/T203Y (E(2)GFP). Halide binding forms a specific nonfluorescent complex generating a substantial drop of the fluorescence via static quenching. Spectroscopic analys...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1914440/ https://ncbi.nlm.nih.gov/pubmed/17434942 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.102319 |
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