Development of a Novel GFP-based Ratiometric Excitation and Emission pH Indicator for Intracellular Studies

We report on the development of the F64L/S65T/T203Y/L231H GFP mutant (E(2)GFP) as an effective ratiometric pH indicator for intracellular studies. E(2)GFP shows two distinct spectral forms that are convertible upon pH changes both in excitation and in emission with pK close to 7.0. The excitation of...

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Detaylı Bibliyografya
Asıl Yazarlar: Bizzarri, Ranieri, Arcangeli, Caterina, Arosio, Daniele, Ricci, Fernanda, Faraci, Paolo, Cardarelli, Francesco, Beltram, Fabio
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Biophysical Society 2006
Konular:
Spectroscopy, Imaging, Other Techniques
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1432127/
https://ncbi.nlm.nih.gov/pubmed/16603505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.074708
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