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Development of a Novel GFP-based Ratiometric Excitation and Emission pH Indicator for Intracellular Studies
We report on the development of the F64L/S65T/T203Y/L231H GFP mutant (E(2)GFP) as an effective ratiometric pH indicator for intracellular studies. E(2)GFP shows two distinct spectral forms that are convertible upon pH changes both in excitation and in emission with pK close to 7.0. The excitation of...
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| Auteurs principaux: | , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Biophysical Society
2006
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1432127/ https://ncbi.nlm.nih.gov/pubmed/16603505 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.074708 |
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