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Short, synthetic and selectively (13)C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes

We report an optimized synthesis of all canonical 2′-O-TOM protected ribonucleoside phosphoramidites and solid supports containing [(13)C(5)]-labeled ribose moieties, their sequence-specific introduction into very short RNA sequences and their use for the structure determination of two protein–RNA c...

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Detalhes bibliográficos
Main Authors: Wenter, Philipp, Reymond, Luc, Auweter, Sigrid D., Allain, Frédéric H.-T., Pitsch, Stefan
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1904103/
https://ncbi.nlm.nih.gov/pubmed/16807315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl427
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