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Characterization of Aberrant Melting Peaks in Unlabeled Probe Assays

An unlabeled probe assay relies on a double-stranded DNA-binding dye to detect and verify target based on amplicon and probe melting. During the development and application of unlabeled probe assays, aberrant melting peaks are sometimes observed that may interfere with assay interpretation. In this...

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Bibliografske podrobnosti
Main Authors: Dames, Shale, Margraf, Rebecca L., Pattison, David C., Wittwer, Carl T., Voelkerding, Karl V.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Investigative Pathology 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1899421/
https://ncbi.nlm.nih.gov/pubmed/17591927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060139
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