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Unlabeled Oligonucleotides as Internal Temperature Controls for Genotyping by Amplicon Melting
Amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, or allele-specific polymerase chain reaction. However, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) requires high-resolution melting and close...
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| Główni autorzy: | , , , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Investigative Pathology
2007
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1899416/ https://ncbi.nlm.nih.gov/pubmed/17591926 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060136 |
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