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The mechanism of addition of pyridoxal 5′-phosphate to Escherichia coli apo-serine hydroxymethyltransferase

Previous studies suggest that the addition of pyridoxal 5′-phosphate to apo-serine hydroxymethyltransferase from Escherichia coli is the last event in the enzyme's folding process. We propose a mechanism for this reaction based on quenched-flow, stopped-flow and rapid-scanning stopped-flow expe...

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Detalhes bibliográficos
Main Authors: Malerba, Francesca, Bellelli, Andrea, Giorgi, Alessandra, Bossa, Francesco, Contestabile, Roberto
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1896279/
https://ncbi.nlm.nih.gov/pubmed/17341210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20061681
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