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APEx 2-hybrid, a quantitative protein–protein interaction assay for antibody discovery and engineering

We have developed a bacterial system for the discovery of interacting proteins that, unlike other two-hybrid technologies, allows for the selection of protein pairs on the basis of affinity or expression. This technology relies on the anchored periplasmic expression (APEx) of one protein (bait) on t...

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書誌詳細
主要な著者: Jeong, Ki Jun, Seo, Min Jeong, Iverson, Brent L., Georgiou, George
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2007
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1895937/
https://ncbi.nlm.nih.gov/pubmed/17494738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0702650104
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