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APEx 2-hybrid, a quantitative protein–protein interaction assay for antibody discovery and engineering
We have developed a bacterial system for the discovery of interacting proteins that, unlike other two-hybrid technologies, allows for the selection of protein pairs on the basis of affinity or expression. This technology relies on the anchored periplasmic expression (APEx) of one protein (bait) on t...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1895937/ https://ncbi.nlm.nih.gov/pubmed/17494738 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0702650104 |
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