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Functional Expression of Recombinant Human Stefin A in Mammalian and Bacterial Cells

Recombinant human cysteine protease inhibitor, stefin A, was expressed in both E. coli and BSC-1 monkey kidney cells utilizing pET and recombinant Vaccinia virus systems, respectively. The expressed protein was purified and analyzed by SDS-PAGE and western blot analysis utilizing a polyclonal antibo...

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Bibliografiske detaljer
Main Authors: Calkins, Catharine C., Dosescu, Julie, Day, Nancy A., Ren, Wei-Ping, Fridman, Rafael, Sloane, Bonnie F., Moin, Kamiar
Format: Artigo
Sprog:Inglês
Udgivet: 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892908/
https://ncbi.nlm.nih.gov/pubmed/17208452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.11.020
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