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Functional Expression of Recombinant Human Stefin A in Mammalian and Bacterial Cells
Recombinant human cysteine protease inhibitor, stefin A, was expressed in both E. coli and BSC-1 monkey kidney cells utilizing pET and recombinant Vaccinia virus systems, respectively. The expressed protein was purified and analyzed by SDS-PAGE and western blot analysis utilizing a polyclonal antibo...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1892908/ https://ncbi.nlm.nih.gov/pubmed/17208452 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.11.020 |
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