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Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes

BACKGROUND: Targeted mutagenesis of the herpesvirus genomes has been facilitated by the use of bacterial artificial chromosome (BAC) technology. Such modified genomes have potential uses in understanding viral pathogenesis, gene identification and characterization, and the development of new viral v...

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Detalhes bibliográficos
Main Authors: Schmeisser, Falko, Weir, Jerry P
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1885250/
https://ncbi.nlm.nih.gov/pubmed/17501993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-7-22
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