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On-column refolding of recombinant chemokines for NMR studies and biological assays

We have applied an efficient solid-phase protein refolding method to the milligram scale production of natively folded recombinant chemokine proteins. Chemokines are intensely studied proteins because of their roles in immune system regulation, response to inflammation, fetal development, and numero...

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Detalhes bibliográficos
Main Authors: Veldkamp, Christopher T., Peterson, Francis C., Hayes, Paulette L., Mattmiller, Jessie E., Haugner, John C., de la Cruz, Norberto, Volkman, Brian F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1868460/
https://ncbi.nlm.nih.gov/pubmed/17071104
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.09.009
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