Precision and Performance Characteristics of Bisulfite Conversion and Real-Time PCR (MethyLight) for Quantitative DNA Methylation Analysis

Assays to measure DNA methylation, which are important in epigenetic research and clinical diagnostics, typically rely on conversion of unmethylated cytosine to uracil by sodium bisulfite. However, no study has comprehensively evaluated the precision and performance characteristics of sodium bisulfi...

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Detalhes bibliográficos
Main Authors: Ogino, Shuji, Kawasaki, Takako, Brahmandam, Mohan, Cantor, Mami, Kirkner, Gregory J., Spiegelman, Donna, Makrigiorgos, G. Mike, Weisenberger, Daniel J., Laird, Peter W., Loda, Massimo, Fuchs, Charles S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2006
Assuntos:
Regular Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1867588/
https://ncbi.nlm.nih.gov/pubmed/16645207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2006.050135
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