Precision and Performance Characteristics of Bisulfite Conversion and Real-Time PCR (MethyLight) for Quantitative DNA Methylation Analysis

Assays to measure DNA methylation, which are important in epigenetic research and clinical diagnostics, typically rely on conversion of unmethylated cytosine to uracil by sodium bisulfite. However, no study has comprehensively evaluated the precision and performance characteristics of sodium bisulfi...

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Main Authors: Ogino, Shuji, Kawasaki, Takako, Brahmandam, Mohan, Cantor, Mami, Kirkner, Gregory J., Spiegelman, Donna, Makrigiorgos, G. Mike, Weisenberger, Daniel J., Laird, Peter W., Loda, Massimo, Fuchs, Charles S.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Investigative Pathology 2006
Ábhair:
Regular Articles
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1867588/
https://ncbi.nlm.nih.gov/pubmed/16645207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2006.050135
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