RET Proto-Oncogene Genotyping Using Unlabeled Probes, the Masking Technique, and Amplicon High-Resolution Melting Analysis

Single bp mutations in the RET proto-oncogene can cause multiple endocrine neoplasia type 2 syndromes. The conventional approach for genotyping RET mutations is sequencing the exons. A closed-tube RET genotyping assay using a saturating DNA dye, unlabeled probes, and amplicon high-resolution melting...

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Bibliografiske detaljer
Main Authors: Margraf, Rebecca L., Mao, Rong, Highsmith, W. Edward, Holtegaard, Leonard M., Wittwer, Carl T.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Investigative Pathology 2007
Fag:
Regular Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1867443/
https://ncbi.nlm.nih.gov/pubmed/17384210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2007.060091
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