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Establishing Acceptance Criteria for Cell-Mediated-Immunity Assays Using Frozen Peripheral Blood Mononuclear Cells Stored under Optimal and Suboptimal Conditions

The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for measuring antigen-specific cellular immune responses. The ability to use frozen peripheral blood mononuclear cells (PBMC) facilitates testing samples in multicenter clinical trials; however, unreliable ELISPOT responses may result i...

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Detalhes bibliográficos
Main Authors: Smith, Jeffrey G., Joseph, Heather R., Green, Tina, Field, Jodie A., Wooters, Melissa, Kaufhold, Robin M., Antonello, Joseph, Caulfield, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1865640/
https://ncbi.nlm.nih.gov/pubmed/17376862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00435-06
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