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Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry
INTRODUCTION: Live peripheral blood mononuclear cells (PBMCs) can be frozen and thawed for later analyses by adding and removing a cryoprotectant, such as dimethyl sulfoxide (DMSO). Laboratories across the world use various procedures, but published evidence of optimal thawing procedures is scarce....
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5665600/ https://ncbi.nlm.nih.gov/pubmed/29091947 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0187440 |
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