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Intracellular cytokine detection by flow cytometry in surface marker-defined human peripheral blood mononuclear T cells
In a recent unit in this series, protocols for the isolation, cryopreservation, thawing, and immunophenotyping of HPBMC isolated from peripheral whole blood using cell surface marker (CSM) staining and multi-color flow cytometry analysis were presented. The current procedure describes the detection...
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| Publicado no: | Curr Protoc Toxicol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5547893/ https://ncbi.nlm.nih.gov/pubmed/28777444 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cptx.26 |
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