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Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells
This protocol describes procedures for the isolation, cryopreservation, and thawing of HPBMC and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This methodology can be used to ensure that cell integrity and phenotype stability is not altered through...
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| Publicat a: | Curr Protoc Toxicol |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5726299/ https://ncbi.nlm.nih.gov/pubmed/29117436 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cptx.31 |
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