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Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells

This protocol describes procedures for the isolation, cryopreservation, and thawing of HPBMC and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This methodology can be used to ensure that cell integrity and phenotype stability is not altered through...

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Detalhes bibliográficos
Publicado no:Curr Protoc Toxicol
Main Authors: Lauer, Fredine T., Denson, Jesse L., Burchiel, Scott W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5726299/
https://ncbi.nlm.nih.gov/pubmed/29117436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cptx.31
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